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To clarify which types of polyubiquitin chain were added to HMOX2 by MITOL, a ubiquitin chain restriction assay using UbiCRest, which utilizes ubiquitin chain-specific deubiquitinating enzymes, was performed 30

At room temperature, media was removed, and cells were washed twice with 0.9% NaCl (aq)

With the rapid development of sequencing technology, significant advancements in our study of intestinal flora have been achieved, and it is also clear that lipid metabolism is closely related to intestinal flora